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SAI International School
Subject- Biology, Class- IX
Topic- The Fundamental Unit of Life
Subtopic- Parts of a Cell, Osmosis & Diffusion
Module- 2
HOME ASSIGNMENT-II (EXPERIMENT)

Experiment-2
Aim of the experiment: To prepare stained temporary mount of human cheek cells
and to record observations and draw labelled diagrams.

Materials Required---
• Glass microscope slides
• Plastic cover slips
• Paper towels or tissue
• Methylene Blue solution (0.5% to 1% (mix approximately 1 part stock
solution with 4 parts of water))
• Plastic pipette or dropper
• Sterile, individually packed cotton swabs

Procedure--

1. Take a clean cotton swab and gently scrape the inside of your mouth.
2. Smear the cotton swab on the centre of the microscope slide for 2 to 3 seconds.
3. Add a drop of methylene blue solution and place a coverslip on top.
Concentrated methylene blue is toxic if ingested. Wear gloves and do NOT
allow children to handle methylene blue solution or have access to the bottle of
solution.