Page 5 - Assignment-Biotechnology-principles and procedure

(a) Name the steps A, B and C.
(b) Give the purpose of each of these steps.
(c) State the contribution of Thermus aquaticus in this process.

21. Study the figure of vector pBR322 given below in which foreign DNA is ligated at
the Bam H1
site of tetracyline resistance gene.

Answer the following questions :
(a) Mention the function of rop.
(b) What will be the selectable marker for this recombinant plasmid and why?
(c) Explain transformation.