Page 3 - Assignment-Biotechnology-principles and procedure

C. Short Answer Questions-II (2 marks each)
8. Name two main steps which are collectively referred to as down streaming process.
Why is this process significant?

9. How does plasmid differ from chromosomal DNA?
10. A bacterial cell is shown in the figure given below. Label the part „A‟ and „B‟. Also
mention the use of part „A‟ in rDNA technology.

11. Mention two classes of restriction enzymes. Suggest their respective roles.
12. In the given process of separation and isolation of DNA fragments, some of the steps
are missing, Complete the missing steps –

D. Short Answer Questions-I (3 marks each)
13. Since DNA is a hydrophillic moelcule, it cannot pass through cell membrane. Name
and explain the technique with which the DNA is forced into
(i) a bacterial cell
(ii) a plant cell
(iii) an animal cell.

14. How will you otbain purified DNA from a cell?