Page 2 - Assignment-Biotechnology-principles and procedure

7. The DNA fragments have sticky ends due to

a. Endonuclease
b. Unpaired bases
c. Calcium ions
d. Free methylation

8. Plasmids are used as cloning vectors for which of the following reasons?

a. Can be multiplied in culture
b. Self-replication in bacterial cells
c. Can be multiplied in laboratories with the help of enzymes
d. Replicate freely outside bacterial cells

9. The human genome project was launched in the year

a. 1980
b. 1973
c. 1990
d. 1989

10. The vaccines prepared through recombinant DNA technology are

a. Third generation vaccines
b. First-generation vaccines
c. Second-generation vaccines
d. None

B. Very Short Answer Questions (1 mark each)
1. A restriction enzyme digests DNA into fragments. Name the technique used to check
the progression of this enzyme and separate DNA fragments.
2. Name two commonly used vectors in genetic engineering.
3. Some enzymes are used to cut DNA into fragements. These are considered as
molecular scissors. What is the name assigned to such enzymes.
4. Write conventional nomenclature of ECORI.
5. A linear DNA fragment and a plasmid has three restriction sites for ECORI how many
fragments will be produced from linear DNA and plasmid respectively.
6. An extra chromosomal segment of circular DNA is used to carry gene of interest into
the host cell. What is the name given to it?
7. Identify the recognition sites in the given sequences at which E.Coli will be cut and
make sticky ends.
5´–GAATTC–3´
3´–CTTAAG–5´