Page 1 - Assignment-Biotechnology-principles and procedure
P. 1
Assignment
A – Multiple Choice Questions (MCQs)
1. Restriction enzymes were discovered by
a. Smith and Nathans
b. Alexander Fleming
c. Berg
d. None
2. Bacteria protect themselves from viruses by fragmenting viral DNA with
a. Ligase
b. Endonuclease
c. Exonuclease
d. Gyrase
3. Klenow fragment is derived from
a. DNA Ligase
b. DNA Pol-I
c. DNA Pol-II
d. Reverse Transcriptase
4. Southern blotting is
a. Attachment of probes to DNA fragments
b. Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
c. Comparison of DNA fragments to two sources
d. Transfer of DNA fragments to electrophoretic gel from cellulose membrane
5. ELISA is
a. Using radiolabelled second antibody
b. Usage of RBCs
c. Using complement-mediated cell lysis
d. Addition of substrate that is converted into a coloured end product
6. The Golden Rice variety is rich in
a. Vitamin C
b. Β-carotene and ferritin
c. Biotin
d. Lysine
