Page 3 - LN-CH-2[2]
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   It is a large diploid cell, dense cytoplasm with prominent nucleus.
                      The MMC undergo meiotic division resulting four haploid megaspores


               Female gametophyte:

                      Out of four megaspores, one megaspore is functional and other three degenerates.
                      The functional megaspore developed into the female gametophyte.
                      Female gametophyte is known as the embryo sac.
                      Development of embryo sac from a single megaspore is called asmonosporic type of embryo sac.
                      The nucleus of the functional megaspore divided by mitotic division to form two nuclei which move to the
                       opposite pole, 2-nucleated embryo sac.
                      Two successive mitotic division leads to formation of 4-nucleate and later 8-nucleate stages of the embryo
                       sac.
                      All mitotic divisions are free nuclear type; karyokinesis is not followed by cytokinesis.
                      Six of the eight nuclei are surrounded by cell walls and organized into cells.
                      Three cells are grouped together at the micropylar end, constitute the egg apparatus.
                      The egg apparatus, in turn consists of two synergids and one egg cell.
                      Synergids have special filiform apparatus, which play an important role in guiding the entry of pollen tube
                       into the synergids.
                      Three cells arranged towards chalazal end are called antipodal cells.
                      The large central cell has two polar nuclei.
                      A typical angiosperm embryo sac at maturity is 8- nucleated and 7-celled.

               Pollination:


                      Transfer of pollen grains from the anther to the stigma of a pistil is termed as pollination.
                      Both male and female gametes are non-motile.


               Kinds of pollination:

               Autogamy:


                      Pollination within same flower.
                      In open and exposed anthers and stigma autogamy is rare.
                      Viola, Oxalis and Commelina produce two types of flowers:
                          o   Chasmogamous: exposed anther and stigma
                          o   Cleistogamous: closed anther and stigma.
                      Cleistogamous  flower  is  invariably autogamous and assured  seed set  even  in  the  absence  of  the
                       pollinator.


               Geitonogamy:

                      Pollination between two flowers of the same plant.
                      Pollination by pollinating agent.
                      Genetically similar to the autogamy.
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