Page 1 - 3.Home_Assignment_II_(Expt &_Activity)_Asexual_Reproduction_-_2

SAI International School
Subject- Biology, Class X
Chapter- How do Organisms Reproduce?
Subtopic- Regeneration, Budding, Vegetative Propagation,
Spore Formation
Module-19
Home Assignment-II (Experiment & Activity)
Lab Work sheet- 1

Experiment-3
Aim of the experiment: To observe the permanent slides of
(a) Binary fission in Amoeba (b) Budding in Yeast

Materials Required:
Compound microscope, permanent slides of binary fission in Amoeba and budding in Yeast.

Procedure:
1. Place the slide under a compound microscope.

2. Focus the slide, first under low power and later under high power of the compound
microscope. and
3. Various stages of binary fission and budding can be carefully examined.

Observation: (a) Binary Fission in Amoeba

1. The nucleus in a matured amoeba elongates. Amitosis is observed, the division of the
nucleus into two nuclei takes place.
2. At the point of fission in the body of the amoeba, a constriction appears in the middle
of cytoplasm.
3. The constriction or furrow turns deeper which separates the cytoplasm into two equal
parts, each with one nucleus.
4. The two daughter cells may separate from each other.

(b) Budding in yeast

1. Protuberance or a small bud-like tiny outgrowth is observed on the parent cell.
2. The nucleus elongates and divides into two daughter nuclei.
3. A constriction appears at the base of the bud and a separating wall is laid down.
4. Repetitive budding leads to the formation of a chain of cells.

Inference:
The prepared slides display asexual reproduction. One individual is involved to produce a
new offspring of its own kind.

Precautions:
1. Slides need to be aligned and focused accurately
2. Sketch out your observation that is observed under a microscope